%0 Journal Article
%T EFFECTS OF HYDROGEN PEROXIDE ON PRODUCING INTRACELLULAR ROS OF WB-344 RAT LIVER EPITHELIAL CELL<br>H2O2致WB-F344细胞内活性氧的产生及机理
%A SHI Guang-pu
%A DU Hong-zhen
%A LIU Zi-wen
%A LIU Xu
%A WU Yuan-de
%A <br>施广璞
%A 杜洪震
%A 刘子文
%A 刘旭
%A 吴元德
%J 生物物理学报
%D 2002
%I 
%X To study the role and mechanism of hydrogen peroxide(H 2 O 2 ) on intracellular reactive oxygen species(ROS) generation of WB-F344 rat liver epithelial cell, DHR123 was used as fluorescence probe to detect the intracellular ROS generation and observed on laser scanning confocal microscope. The results showed that: (1)The generation of intracellular ROS of the cell stimulated by H 2 O 2 increased slowly and gradually. (2)The intracellular ROS production stimulated by H 2 O 2 decreased when pretreated with ROS scavenger NAC. (3)There were no production of intracellular ROS pretreated with 2-Aminop-urine, a broader spectrum protein kinase inhibitor, or pretreated with Tyrphostin 25, a specific inhibitor of tyrosine protein kinase, or with Bisindolylmaleimide I, a PKC inhibitor. (4)The intracellular ROS could still be generated but the level decreased slightly by incubated in D-Hanks solution containing EG-TA, the chelate of extracellular Ca 2+ . (5)There were no generation of intracellular ROS pretreated with NAC and incubated WB cell in D-Hanks solution containing EGTA. These results suggested that the pathway of intracellular ROS generation stimulated by H 2 O 2 may be related to PKC, TPK and Ca 2+ signal transduction.
%K Hydrogen
%K peroxide
%K Intracellular
%K reactive
%K oxygen
%K species
%K generation
%K Signal
%K transduction<br>过氧化氢
%K 胞内活性氧
%K 信号转导
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=2532F00DB4B52836&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=E158A972A605785F&sid=8C5DE51F0A009A0F&eid=4D4C81DBA842B7BD&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=4&reference_num=18