%0 Journal Article
%T Studies on the differences of gene expression in Scophthalmus maximus L. muscle of large versus small scaled individuals<br>大菱鲆肌肉组织中的基因差异表达与体长性状的相关性研究
%A WANG Hui
%A XIE Xiang-Lin
%A LIU Xian-Zhong
%A LI Rong-Ling
%A WANG Dan-Chen
%A <br>王慧
%A 解相林
%A 刘贤忠
%A 李荣岭
%A 王丹晨
%J 水生生物学报
%D 2007
%I 
%X In order to understand the molecular basis of fish growth and development,the technique of mRNA differential display was used to analyze the differences in gene expression of muscle tissue in Scophthalmus maximus L muscle of large versus small scaled individuals.Only bands that can be repeated in polyacrylamide gel electrophoresis of duplicate PCR products were used for statistic analysis.Our results showed that the differential expressed gene between the 2 fish flocks was very obvious.18 kinds of primer subsets amplified 527 clear bands of cDNA fragment.In them,the number of differential expressed cDNA fragment is 16(3.04%);both fish flocks coincident expression 506(96.02%) bands.The differentially expressed cDNA fragments must be related to the growth and development of fish.The screen of these differentially expressed genes will help us to understand the molecular mechanism in Scophthalmus maximus L.growth and development.
%K Scophthalmus maximus L
%K Muscle
%K mRNA Differential Display
%K Differential expressed cDNA<br>技术应用的综合示范"水产子课题(04033810)
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=C41540C6F851C550&yid=A732AF04DDA03BB3&vid=4AD960B5AD2D111A&iid=E158A972A605785F&sid=CA4FD0336C81A37A&eid=CF0706A3E35031F6&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=0&reference_num=11