%0 Journal Article
%T SURFACE HYDROPHOBICITY OF CHICKEN HEART APOCYTOCHROME C WITH DIFFERENT UNFOLDED STATES<br>不同解折叠状态鸡心脱血红素细胞色素c分子的表面性质
%A Tong Junchao
%A Yang Fuyu
%A <br>童俊超
%A 杨福愉
%J 生物物理学报
%D 1994
%I 
%X The ability of apocytochrome c to move across lipid bilayer is dependent on its folding states. Chicken heart apocytochrome c has relatively stronger tendency to fold spontaneously,and its different unfolded states (C1, C2, C3) could be obtained in vitro. The surface hydrophobicity of chicken heart apocytochrome c with different unfolded states has been compared by hydrophobic chromatography and by assessing their ability to bind a hydrophobic probe, 1,8-ANS. In addition, the fluorescence resonance energy transfer between the tryptophan residue and the bound 1,8-ANS has also been studied. The results showed that the partially folded chicken heart apocytochrome c has a highly dynamic hydrophobic core and also tends to aggregate, which is consistent with its folding tendency. The implications in the import of apocytochrome c into mitochondria and in its interactions with phospholipids have been discussed.
%K Chicken heart apocytochrome c Unfolded states Surface hydrophobicity 1
%K 8-ANS Hydrophobic chromatography<br>鸡心脱血红素细胞色素c，解折叠状态，表面疏水性，1，8—ANS，疏水层析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=921269908311B5AE5C684386BFDF0100&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=E158A972A605785F&sid=41A78CBB5BAB6860&eid=6A9657F54F754BF6&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=2