%0 Journal Article
%T Evaluation of Flavonoids and Total Phenolic Contents of Stem Bark and Leaves of Parkia biglobosa (Jacq.) Benth. (Mimosaceae)-Free Radical Scavenging and Antimicrobial Activities
%A H. Millogo-Kone
%A M. Lompo
%A F. Kini
%A S. Asimi
%A I.P. Guissou
%A O. Nacoulma
%J Research Journal of Medical Sciences
%D 2012
%I 
%X The total phenolics and the flavonoids contents of stem barks and leaves of Parkia biglobosa harvested at different periods of the year, on 2 different areas of Burkina Faso (Yako and L¨¨na), were determined. The total phenolics were extracted according to Harborne s method and also by the means of a soxhlet apparatus. The determination of the phenolics contents was conducted with 8 extracts using tannic acid as standard. The flavonoids contents of the 8 extracts were also determined, using quercetin as a reference substance. The results showed that the total phenolics contents vary significantly all year round at the same area and also from L¨¨na to Yako. They also revealed that the flavonoids contents of the leaves harvested at Yako ranged from 1.49-1.80% throughout the year. The results also confirmed that the leaves contain more flavonoids than the barks. Antioxidant activity was determined by the means of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. The results showed effectiveness with a radical inhibition ranging from 25.53¡À3.56-55.449¡À4.53 mg mL-1. The antibacterial assays on gram positive Staphylococcus aureus showed that the hydroalcoholic extract of stem bark harvested in May at Yako was the most effective of all the extracts.
%U http://www.medwellonline.net/abstract/?doi=rjmsci.2009.70.74