%0 Journal Article
%T Genome-Wide Profiling of miRNAs and Other Small Non-Coding RNAs in the Verticillium dahliae¨CInoculated Cotton Roots
%A Zujun Yin
%A Yan Li
%A Xiulan Han
%A Fafu Shen
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0035765
%X MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are short (19¨C25 nucleotides) non-coding RNA molecules that have large-scale regulatory effects on development and stress responses in plants. Verticillium wilt is a vascular disease in plants caused by the fungal pathogen Verticillium dahliae. The objective of this study is to investigate the transcriptional profile of miRNAs and other small non-coding RNAs in Verticillium¨Cinoculated cotton roots. Four small RNA libraries were constructed from mocked and infected roots of two cotton cultured species which are with different Verticillium wilt tolerance (¡®Hai-7124¡¯, Gossypium barbadense L., a Verticillium-tolerant cultivar, and ¡®Yi-11¡¯, Gossypium hirsutum L. a Verticillium-sensitive cultivar). The length distribution of obtained small RNAs was significantly different between libraries. There were a total of 215 miRNA families identified in the two cotton species. Of them 14 were novel miRNAs. There were >65 families with different expression between libraries. We also identified two trans-acting siRNAs and thousands of endogenous siRNA candidates, and hundred of them exhibited altered expression after inoculation of Verticillium. Interesting, many siRNAs were found with a perfect match with retrotransposon sequences, suggested that retrotransposons maybe one of sources for the generation of plant endogenous siRNAs. The profiling of these miRNAs and other small non-coding RNAs lay the foundation for further understanding of small RNAs function in the regulation of Verticillium defence responses in cotton roots.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0035765