%0 Journal Article
%T ATF3 Plays a Key Role in Kdo2-Lipid A-Induced TLR4-Dependent Gene Expression via NF-¦ÊB Activation
%A Eun-Young Kim
%A Hye Young Shin
%A Joo-Young Kim
%A Dong-Gun Kim
%A Yong-Min Choi
%A Hyuk-Kwon Kwon
%A Dong-Kwon Rhee
%A You-Sun Kim
%A Sangdun Choi
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0014181
%X Activating transcription factor 3 (ATF3) is a negative regulator of proinflammatory cytokine expression in macrophages, and ATF3 deficient mice are more susceptible to endotoxic shock. This study addresses the role of ATF3 in the Kdo2-Lipid A-induced Toll-like receptor 4 (TLR4) signaling pathway in mouse embryonic fibroblasts (MEF). Kdo2-Lipid A upregulates ATF3 expression in wild type MEF cells and induces both nuclear factor kappa B (NF-¦ÊB) and c-Jun N-terminal kinase (JNK) activation via the TLR4 signaling pathway, while neither of these pathways is activated in ATF3-/- MEF cells. Interestingly, in contrast to Kdo2-Lipid A, the activation of both NF-¦ÊB and JNK by TNF-¦Á was normal in ATF3-/- MEF cells.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0014181