%0 Journal Article
%T Fluorescent Protein-Based Methods for On-Plate Screening of Gene Insertion
%A Stanley S. C. Wong
%A Kevin Truong
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0014274
%X Unlike the commonly used method of blue-white screening for gene insertion, a fluorescent protein-based screening method offers a gain-of-function screening process without using any co-factors and a gene fusion product with a fluorescent protein reporter that is further useful in cell imaging studies. However, complications related to protein-folding efficiencies of the gene insert in fusion with fluorescent protein reporters prevent effective on-plate bacterial colony selection leading to its limited use.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0014274