%0 Journal Article
%T Enantioseparation of Nadifloxacin by High performance liquid Chromatography
%J Advances in Analytical Chemistry
%@ 2163-2847
%D 2012
%I 
%R 10.5923/j.aac.20120204.01
%X A rapid isocratic chiral HPLC method has been developed for the separation of R-Nadifloxacin from S-Nadifloxacin. Good resolution viz. Rs> 4.0 between R- and S- forms of Nadifloxacin was achieved by RP-HPLC using endcappedC18 stationary phase and chiral mobile phase. Chirality to the mobile phase was imparted with addition of ¦Â-CD in phosphate buffer with EDTA.Column temperature was 45¡ãC and flow rate was kept 1.5 mL min-1.The elution was monitored by UV-vis detector at ¦Ë-290 nm. The calibration curve showed excellent linearity over concentration range 0.040-20 ¦Ìg mL-1.This method was further used to determine the amount of R-Nadifloxacin in pure and active pharmaceutical ingredient of S-Nadifloxacin and is capable to quantitate and detect R-Nadifloxacin to the levels of 0.040¦Ìg mL-1and 0.020¦Ìg mL-1 respectively. The average recovery of R-Nadifloxacin was 99.09 %. The method is better than the already reported one for the enantioseparation of the Nadifloxacin.
%K Enantioseparation
%K Chiral Mobile Phase Additive
%K (R
%K S)-Nadifloxacin
%K ¦Â-Cyclodextrin
%U http://article.sapub.org/10.5923.j.aac.20120204.01.html