%0 Journal Article
%T Multi-Drug Resistance in Health Care-Associated Bacteremia in Intensive Care Units at King Fahad Specialized Hospital, Buraidah, Saudi Arabia
%J Journal of Microbiology Research
%@ 2166-5931
%D 2012
%I 
%R 10.5923/j.microbiology.20120206.01
%X Health care-associated bacteremia cause important morbidity and mortality and have a considerable impact on healthcare costs. Infections caused by extended spectrum beta-lactamase producing bacteria are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. Objectives: To analyze health care-associated bacteremia in ICUs of King Fahad Specialized Hospital and to assess ESBL production in the isolated Gram negative bacteria. Methods: This study included 519 patients. Their blood samples were collected for blood culture. The isolates were identified and antibiotic sensitivity tests were performed. The type of B-lactamase gene was determined by polymerase chain reaction (PCR). Results: The rate of health care-associated bacteremia was 9.8%. Gram positive organisms were detected in 67.8 %; methithilin resistant Staph.aureus (MRSA) was the most prevalent (17.8 %). Gram negative bacilli were detected in 30.6 %. E.coli was the most common (12.9%). The production of extended spectrum ¦Â-lactamase (ESBL) enzyme was positive in 84.2% of the isolated Gram negative isolates. Temoniera (TEM) was the main type of ¦Â-lactamase. Conclusion: The isolation of multi-drug resistant bacteria and ESBL producing Gram negative organisms in ICU patients resulted in a greater awareness of implementation of new rules for microbiological screening and infection control measures.
%K Health Care-Associated Bacteremia
%K Klebsiella Pneumoniae
%K Methicillin Resistant Staph Aureus
%K Extended Spectrum Beta-lactamase
%U http://article.sapub.org/10.5923.j.microbiology.20120206.01.html