%0 Journal Article
%T DNA Manipulation in Biological Liquid Environment Based on Atomic Force Microscopy
%J Nanoscience and Nanotechnology
%@ 2163-2588
%D 2012
%I 
%R 10.5923/j.nn.20120204.04
%X During the recent years, one of the most important progresses in nano world was the development of methods for direct access to single molecules on the nanometer scale. The most significant ones have been the scanning probe microscopes such as the atomic force microscope (AFM). AFM is a versatile tool for imaging, force measurement and manipulation of different nanoparticles including proteins, DNA, and living cells. This work is focused on manipulation of biological cells specially DNA in biological liquid and is analyzed dynamics of the spherical nanoparticle manipulation based on Atomic Force Microscopy (AFM). In previous investigations, pushing of the gold nanoparticle using AFM was modeled in air and liquid and the dynamic behavior of tip and nanoparticle was investigated. The problem is simulated for DNA nanoparticle pushing on a silicon substrate in biological liquid using AFM. The biological liquid is ethanol in all the parts, but a comparison has been made between three liquids (ethanol, methanol and blood) in order to investigate the effect of environment on the results. The results show that, for DNA (as compared to gold), the required manipulation force and time for nanoparticle sliding and rolling increase by 17.9 and 18.9%, for sliding and 24.7 and 24.2% for rolling, respectively. The results also make it clear that in comparison to gold, there is an increase in the type of particle movement; in case of gold, the particles with radiuses larger than 480 nm starts to rolling before sliding, while for DNA it happens for particles with radiuses larger than 530 nm. The comparison between three important contact theories (JKR, DMT and Hertz) shows that there is a small difference between the critical times and forces of them.
%K Nano-manipulation
%K Pushing
%K Dynamic Model
%K DNA Molecules
%K Atomic Force Microscopy
%U http://article.sapub.org/10.5923.j.nn.20120204.04.html