%0 Journal Article
%T Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells
%A Kim CH
%A Chung CW
%A Choi KH
%A Yoo JJ
%A Kim DH
%A Jeong YI
%A Kang DH
%J International Journal of Nanomedicine
%D 2011
%I 
%R http://dx.doi.org/10.2147/IJN.S21395
%X t of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells Original Research (4114) Total Article Views Authors: Kim CH, Chung CW, Choi KH, Yoo JJ, Kim DH, Jeong YI, Kang DH Published Date June 2011 Volume 2011:6 Pages 1357 - 1363 DOI: http://dx.doi.org/10.2147/IJN.S21395 Cy Hyun Kim1,2, Chung-Wook Chung1, Kyung Ha Choi1, Jin-Ju Yoo1, Do Hyung Kim1,2, Young-IL Jeong1, Dae Hwan Kang1,2 1National Research and Development Center for Hepatobiliary Cancer, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea; 2School of Medicine, Pusan National University, Yangsan, Republic of Korea The first two authors contributed equally to this work. Abstract: Cancer cells have been reported to exhibit an enhanced capacity for protoporphyrin IX (PpIX) synthesis facilitated by the administration of 5-aminolevulinic acid (ALA). We investigated the effect of ALA-based photodynamic therapy (PDT) on human cholangiocarcinoma cells (HuCC-T1). Since protoporphyrin IX (PpIX), a metabolite of ALA, can produce reactive oxygen species (ROS) under irradiation and then induce phototoxicity, ALA-based PDT is a promising candidate for the treatment of cholangiocarcinoma. When various concentrations of ALA (0.05¨C2 mM) were used to treat HuCC-T1 cells for 6 or 24 hours, the intracellular PpIX level increased according to the ALA concentration and treatment time. Furthermore, an increased amount of PpIX in HuCC-T1 cells induced increased production of ROS by irradiation, resulting in increased phototoxicity.
%K ALA-based photodynamic therapy
%K HuCC-T1
%K protoporphyrin IX
%K ROS
%U https://www.dovepress.com/effect-of-5-aminolevulinic-acid-based-photodynamic-therapy-via-reactiv-peer-reviewed-article-IJN