%0 Journal Article
%T How can micelle systems be rebuilt by a heating process?
%A Silva-Filho MA
%A Siqueira SDVS
%A Freire LB
%A ArašČjo IB
%A Silva KGH
%A Medeiros AC
%A ArašČjo-Filho I
%A Oliveira AG
%A Egito EST
%J International Journal of Nanomedicine
%D 2012
%I 
%R http://dx.doi.org/10.2147/IJN.S25761
%X w can micelle systems be rebuilt by a heating process? Original Research (1924) Total Article Views Authors: Silva-Filho MA, Siqueira SDVS, Freire LB, ArašČjo IB, Silva KGH, Medeiros AC, ArašČjo-Filho I, Oliveira AG, Egito EST Published Date January 2012 Volume 2012:7 Pages 141 - 150 DOI: http://dx.doi.org/10.2147/IJN.S25761 Received: 02 September 2011 Accepted: 10 October 2011 Published: 12 January 2012 Miguel Adelino da Silva-Filho1, Scheyla Daniela Vieira da Silva Siqueira1, Larissa Bandeira Freire1, Ivonete Batista de ArašČjo2, Kšąttya Gyselle de Holanda e Silva2, Aldo da Cunha Medeiros3, Irami ArašČjo-Filho3, Anselmo Gomes de Oliveira4, Eryvaldo Sšźcrates Tabosa do Egito2 1Dispersed Systems Laboratory, Federal University of Rio Grande do Norte (UFRN), Natal, Rio Grande do Norte, Brazil; 2Department of Pharmacy, UFRN, Natal, Rio Grande do Norte, Brazil; 3Department of Experimental Surgery, UFRN, Natal, Rio Grande do Norte, Brazil; 4Department of Drugs and Medicines, College of Pharmaceutical Sciences (UNESP), Araraquara, S o Paulo, Brazil Abstract: The aim of this work was to evaluate how an aqueous micellar system containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg Ą€ L-1, 5 mg Ą€ L-1, 0.5 mg Ą€ L-1, and 0.05 mg Ą€ L-1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K+) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the efficacy of AmB-DOC and AmB-DOC-H were assessed by K+ leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg Ą€L-1). For K+ leakage, both AmB-DOC and AmB-DOC-H showed a similar profile for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profile of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections.
%K micelles
%K nanotechnology
%K pre-heated amphotericin B
%K super-aggregates
%U https://www.dovepress.com/how-can-micelle-systems-be-rebuilt-by-a-heating-process-peer-reviewed-article-IJN