%0 Journal Article
%T Nanostructured delivery system for zinc phthalocyanine: preparation, characterization, and phototoxicity study against human lung adenocarcinoma A549 cells
%A Mariana da Volta Soares
%A Mainara Rangel Oliveira
%A Elisabete Pereira dos Santos
%A et al
%J International Journal of Nanomedicine
%D 2011
%I 
%R http://dx.doi.org/10.2147/IJN.S15860
%X nostructured delivery system for zinc phthalocyanine: preparation, characterization, and phototoxicity study against human lung adenocarcinoma A549 cells Original Research (5230) Total Article Views Authors: Mariana da Volta Soares, Mainara Rangel Oliveira, Elisabete Pereira dos Santos, et al Published Date January 2011 Volume 2011:6 Pages 227 - 238 DOI: http://dx.doi.org/10.2147/IJN.S15860 Mariana da Volta Soares1, Mainara Rangel Oliveira1, Elisabete Pereira dos Santos1, Lycia de Brito Gitirana2, Gleyce Moreno Barbosa3, Carla Holandino Quaresma3, Eduardo Ricci-J迆nior1 1Department of Medicines, Laborat車rio de Desenvolvimento Gal那nico (LADEG), Faculty of Pharmacy, 2Laboratory of Animal and Comparative Histology, Glycobiology Research Program, Institute of Biomedical Science, 3Department of Medicines, Laborat車rio Multidisciplinar de Ci那ncias Farmac那uticas, Faculty of Pharmacy, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil Abstract: In this study, zinc phthalocyanine (ZnPc) was loaded onto poly-汍-caprolactone (PCL) nanoparticles (NPs) using a solvent emulsification每evaporation method. The process yield and encapsulation efficiency were 74.2% ㊣ 1.2% and 67.1% ㊣ 0.9%, respectively. The NPs had a mean diameter of 187.4 ㊣ 2.1 nm, narrow distribution size with a polydispersity index of 0.096 ㊣ 0.004, zeta potential of -4.85 ㊣ 0.21 mV, and spherical shape. ZnPc has sustained release, following Higuchi＊s kinetics. The photobiological activity of the ZnPc-loaded NPs was evaluated on human lung adenocarcinoma A549 cells. Cells were incubated with free ZnPc or ZnPc-loaded NPs for 4 h and then washed with phosphate-buffered saline. Culture medium was added to the wells containing the cells. Finally, the cells were exposed to red light (660 nm) with a light dose of 100 J/cm2. The cellular viability was determined after 24 h of incubation. ZnPc-loaded NPs and free photosensitizer eliminated about 95.9% ㊣ 1.8% and 28.7% ㊣ 2.2% of A549 cells, respectively. The phototoxicity was time dependent up to 4 h and concentration dependent at 0每5 米g ZnPc. The cells viability decreased with the increase of the light dose in the range of 10每100 J/cm2. Intense lysis was observed in the cells incubated with the ZnPc-loaded NPs and irradiated with red light. ZnPc-loaded PCL NPs are the release systems that promise photodynamic therapy use.
%K photosensitizer
%K nanoparticles
%K photodynamic therapy
%K lung cancer
%K phototoxicity
%K biodistribution
%U https://www.dovepress.com/nanostructured-delivery-system-for-zinc-phthalocyanine-preparation-cha-peer-reviewed-article-IJN