%0 Journal Article
%T Electrospun chitosan-graft-poly (¦Å-caprolactone)/poly (¦Å-caprolactone) nanofibrous scaffolds for retinal tissue engineering
%A Honglin Chen
%A Xianqun Fan
%A Jing Xia
%A et al
%J International Journal of Nanomedicine
%D 2011
%I 
%R http://dx.doi.org/10.2147/IJN.S17057
%X trospun chitosan-graft-poly (¦Å-caprolactone)/poly (¦Å-caprolactone) nanofibrous scaffolds for retinal tissue engineering Original Research (5205) Total Article Views Authors: Honglin Chen, Xianqun Fan, Jing Xia, et al Published Date February 2011 Volume 2011:6 Pages 453 - 461 DOI: http://dx.doi.org/10.2147/IJN.S17057 Honglin Chen1,2, Xianqun Fan1, Jing Xia1, Ping Chen1, Xiaojian Zhou1, Jin Huang2, Jiahui Yu2, Ping Gu1 1Department of Ophthalmology, Shanghai Ninth People¡¯s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China; 2Institutes for Advanced Interdisciplinary Research, East China Normal University, Shanghai, China Abstract: A promising therapy for retinal diseases is to employ biodegradable scaffolds to deliver retinal progenitor cells (RPCs) for repairing damaged or diseased retinal tissue. In the present study, cationic chitosan-graft-poly(¦Å-caprolactone)/polycaprolactone (CS-PCL/PCL) hybrid scaffolds were successfully prepared by electrospinning. Characterization of the obtained nanofibrous scaffolds indicated that zeta-potential, fiber diameter, and the content of amino groups on their surface were closely correlated with the amount of CS-PCL in CS-PCL/PCL scaffolds. To assess the cell¨Cscaffold interaction, mice RPCs (mRPCs) were cultured on the electrospun scaffolds for 7 days. In-vitro proliferation assays revealed that mRPCs proliferated faster on the CS-PCL/PCL (20/80) scaffolds than the other electrospun scaffolds. Scanning electron microscopy and the real-time quantitative polymerase chain reaction results showed that mRPCs grown on CS-PCL/PCL (20/80) scaffolds were more likely to differentiate towards retinal neurons than those on PCL scaffolds. Taken together, these results suggest that CS-PCL/PCL(20/80) scaffolds have potential application in retinal tissue engineering.
%K electrospun
%K retinal progenitor cells
%K proliferation
%K differentiation
%K tissue engineering
%U https://www.dovepress.com/electrospun-chitosan-graft-poly-epsilon-caprolactonepoly-epsilon-capro-peer-reviewed-article-IJN