%0 Journal Article
%T Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
%A Zhu XM
%A Wang YX
%A Leung KCF
%A Lee SF
%A Zhao F
%A Wang DW
%A Lai JMY
%A Wan C
%A Cheng CHK
%A Ahuja AT
%J International Journal of Nanomedicine
%D 2012
%I 
%R http://dx.doi.org/10.2147/IJN.S28316
%X hanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines Original Research (3640) Total Article Views Authors: Zhu XM, Wang YX, Leung KCF, Lee SF, Zhao F, Wang DW, Lai JMY, Wan C, Cheng CHK, Ahuja AT Published Date February 2012 Volume 2012:7 Pages 953 - 964 DOI: http://dx.doi.org/10.2147/IJN.S28316 Received: 16 November 2011 Accepted: 29 December 2011 Published: 21 February 2012 Xiao-Ming Zhu1, Yi-Xiang J Wang1, Ken Cham-Fai Leung2,3, Siu-Fung Lee2, Feng Zhao1, Da-Wei Wang2, Josie MY Lai4, Chao Wan4, Christopher HK Cheng4, Anil T Ahuja1 1Department of Imaging and Interventional Radiology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR; 2Institute of Molecular Functional Materials and Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR; 3Institute of Creativity and Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR; 4School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong Purpose: To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO2-NH2)-coated superparamagnetic iron oxide (SPIO@SiO2-NH2) nanoparticles with three other types of SPIO nanoparticles coated with SiO2 (SPIO@SiO2), dextran (SPIO@dextran), or bare SPIO in mammalian cell lines. Materials and methods: Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7¨C15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbecco¡¯s modified Eagle¡¯s medium (DMEM) with 4.5 ¦Ìg/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic resonance imaging of MSC pellets after SPIO labeling was performed at 3 T. The effect of each SPIO nanoparticle on the cell viability of RAW 264.7 (mouse monocyte/macrophage) cells was also evaluated. Results: Transmission electron microscopy demonstrated surface coating with SiO2-NH2, SiO2, and dextran prevented SPIO nanoparticle aggregation in DMEM culture medium. MCF-7, MDA-MB-231, and HT-29 cells failed to show notable iron uptake. For all the remaining six cell lines, Prussian blue staining and intracellular iron quantification demonstrated that SPIO@SiO2-NH2 nanoparticles had the highest cellular uptake efficiency. SPIO@SiO2-NH2, bare SPIO, and SPIO@dextran nanoparticles did not affect RAW 264.7 cell viability up to 200 ¦Ìg Fe/mL, while SPIO@SiO2 reduced RAW 264.7 cell viability from 10 to 200 ¦Ìg Fe/mL in a dose-dependent manner. Conclusion: Cellular uptake efficiency of SPIO nanoparticles depends on both the cell type and SPIO surface characteristics. Aminosilane su
%K magnetic nanoparticles
%K SPIO
%K iron oxide
%K surface coating
%K cellular uptake
%U https://www.dovepress.com/enhanced-cellular-uptake-of-aminosilane-coated-superparamagnetic-iron--peer-reviewed-article-IJN