%0 Journal Article
%T Mapping protein–RNA interactions
%A Vaughan R
%A Running W
%A Qi R
%A Kao CC
%J Virus Adaptation and Treatment
%D 2012
%I Dove Medical Press
%R http://dx.doi.org/10.2147/VAAT.S31299
%X pping protein–RNA interactions Review (2977) Total Article Views Authors: Vaughan R, Running W, Qi R, Kao CC Published Date May 2012 Volume 2012:4 Pages 29 - 41 DOI: http://dx.doi.org/10.2147/VAAT.S31299 Received: 29 February 2012 Accepted: 29 March 2012 Published: 02 May 2012 Robert Vaughan, William Running, Rongsu Qi, C Cheng Kao The Biochemistry Interdisciplinary Program, Indiana University, Bloomington, IN, USA Abstract: There is a significant need to develop approaches for rapid and accurate mapping of protein–ribonucleic acid (RNA) interactions, especially to complement structure-based methods. Approaches using mass spectrometry to map regions in proteins that contact RNA have now been established. These include a reversible crosslinking affinity purification method, residue-specific modification interference assay, and photoactivatable crosslinking and mass spectrometry. Novel methods to identify nucleotides within RNA that contact proteins using photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation are also available. In combination, these methods should generate results that will lead to more specific hypotheses concerning the biological properties of protein–RNA interactions. This review summarizes some recent advances in select assays useful for mapping protein–RNA interactions.
%K hepatitis C virus
%K positive-strand RNA virus
%K reversible crosslinking
%K RNA binding
%K mass spectrometry
%U https://www.dovepress.com/mapping-proteinndashrna-interactions-peer-reviewed-article-VAAT