%0 Journal Article
%T Sustained-release G-CSF microspheres using a novel solid-in-oil-in-oil-in-water emulsion method
%A Liu G
%A Hong X
%A Jiang M
%A Yuan W
%J International Journal of Nanomedicine
%D 2012
%I 
%R http://dx.doi.org/10.2147/IJN.S33993
%X stained-release G-CSF microspheres using a novel solid-in-oil-in-oil-in-water emulsion method Original Research (2343) Total Article Views Authors: Liu G, Hong X, Jiang M, Yuan W Published Date August 2012 Volume 2012:7 Pages 4559 - 4569 DOI: http://dx.doi.org/10.2147/IJN.S33993 Received: 18 May 2012 Accepted: 14 June 2012 Published: 17 August 2012 Guang Liu,1 Xiaoyun Hong,2 Mier Jiang,1 Weien Yuan2 1Department of Vascular Surgery, Shanghai Ninth People¡¯s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China Background: The main treatments for cancers are still chemotherapy and radiotherapy for intermediate-stage cancer and terminal cancer. However, the therapeutic methods often result in a decreased neutrophilic granulocyte count and other side effects. In this study, in order to improve the neutrophilic granulocyte levels in the blood after radiotherapy and chemotherapy, we developed a sustained-release granulocyte colony¨Cstimulating factor (G-CSF) microsphere formulation using a novel solid-in-oil-in-oil-in-water (S/O/O/W) emulsification method. Methods: G-CSF was loaded into dextran nanoparticles by freezing-induced phase separation, and then the G-CSF¨Cloaded nanoparticles were encapsulated into sustained-release poly(lactic-co-glycolic acid) microspheres using S/O/O/W emulsification. The control microspheres were also prepared through W/O/W emulsification. The performance of the two microsphere formulations was investigated both in vitro and in vivo. Results: The microspheres for the controlled release of G-CSF in a zero-order or near-zero-order pattern were provided for 2 weeks. The in vitro cumulative G-CSF release kept over 90% of its bioactivity, which was proved by a NFS-60 cell line growth assay. The microspheres of the control group fabricated by W/O/W emulsification maintained less then half of its bioactivity. The in vivo efficacy of microspheres made using the S/O/O/W method was higher than those using the W/O/W method. Conclusion: These results suggested that the microspheres prepared by the S/O/O/W method had increased neutrophil activity compared to those prepared by W/O/W.
%K microspheres
%K bioactivity
%K G-CSF
%K stability
%K sustained release
%U https://www.dovepress.com/nbspsustained-release-g-csf-microspheres-using-a-novel-solid-in-oil-in-peer-reviewed-article-IJN