%0 Journal Article
%T Impact of ghrelin on vitreous cytokine levels in an experimental uveitis model
%A Turgut B
%A Gul FC
%A Dagli F
%A Ilhan N
%A Ozgen M
%J Drug Design, Development and Therapy
%D 2013
%I Dove Medical Press
%R http://dx.doi.org/10.2147/DDDT.S39453
%X ct of ghrelin on vitreous cytokine levels in an experimental uveitis model Original Research (1133) Total Article Views Authors: Turgut B, Gul FC, Dagli F, Ilhan N, Ozgen M Published Date January 2013 Volume 2013:7 Pages 19 - 24 DOI: http://dx.doi.org/10.2147/DDDT.S39453 Received: 23 October 2012 Accepted: 29 November 2012 Published: 10 January 2013 Burak Turgut,1 Fatih Cem G¨¹l,1 Ferda Da li,2 Nevin Ilhan,3 Metin  zgen4 1Department of Ophthalmology, School of Medicine, Firat University, Elazig, 2Department of Pathology, School of Medicine,  n n¨¹ University, Malatya, 3Department of Biochemistry, School of Medicine, Firat University, Elazig, 4Department of Rheumatology, School of Medicine,  n n¨¹ University, Malatya, Turkey Background: The purpose of this study was to investigate the effect of intraperitoneal ghrelin on vitreous levels of interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha (TNF-¦Á) and to compare its effects with those of intraperitoneal infliximab in an experimental uveitis model. Methods: Twenty-four male rats were assigned to four groups of six rats in each. All the rats, except for those in group 1 (controls), were injected intravitreally with concanavalin A to induce experimental uveitis. Rats in group 2 (sham) were not given any treatment after uveitis was induced. Rats in group 3 were given intraperitoneal infliximab 0.5 mg/100 mL on days 0, 1, 3, 5, and 7 following induction of uveitis on day 14 of the study. Rats in group 4 were given intraperitoneal ghrelin 10 ng/kg/day for 7 days following induction of uveitis. On day 21 of the study, enucleated globes were subjected to histopathologic examination. Vitreous levels of IL-1, IL-6, and TNF-¦Á were measured by enzyme-linked immunosorbent assay. Results: Vitreous levels of IL-1, IL-6, and TNF-¦Á were significantly increased in the sham group relative to the control group (P < 0.05), but showed a significant decrease in the group treated with infliximab (P < 0.05). Cytokine levels also decreased in the ghrelin-treated group, but the decrease was not statistically significant (P > 0.05). Conclusion: Ghrelin failed to decrease the IL-1, IL-6, and TNF-¦Á levels that play a critical role in the pathogenesis of uveitis.
%K uveitis
%K tumor necrosis factor-alpha
%K interleukin-1
%K interleukin-6
%K infliximab
%K ghrelin
%U https://www.dovepress.com/impact-of-ghrelin-on-vitreous-cytokine-levels-in-an-experimental-uveit-peer-reviewed-article-DDDT