%0 Journal Article
%T Gelatin tannate reduces the proinflammatory effects of lipopolysaccharide in human intestinal epithelial cells
%A Frasca G
%A Cardile V
%A Puglia C
%A Bonina C
%A Bonina F
%J Clinical and Experimental Gastroenterology
%D 2012
%I Dove Medical Press
%R http://dx.doi.org/10.2147/CEG.S28792
%X tin tannate reduces the proinflammatory effects of lipopolysaccharide in human intestinal epithelial cells Original Research (2010) Total Article Views Authors: Frasca G, Cardile V, Puglia C, Bonina C, Bonina F Published Date May 2012 Volume 2012:5 Pages 61 - 67 DOI: http://dx.doi.org/10.2147/CEG.S28792 Received: 03 December 2011 Accepted: 16 February 2012 Published: 09 May 2012 Giuseppina Frasca1, Venera Cardile1, Carmelo Puglia2, Claudia Bonina2, Francesco Bonina2 1Department of Biomedical Sciences, (Physiology), 2Department of Drug Sciences, University of Catania, Catania, Italy Background: Gelatin tannate is a mixture of tannic acid and gelatin. Tannic acid has astringent properties, due to its capacity to form protein¨Cmacromolecular complexes, as well as antibacterial and antioxidant properties. However, little is known about its anti-inflammatory properties. Purpose: To evaluate the anti-inflammatory activity of gelatin tannate by quantifying the suppression of key molecules produced during inflammatory events in lipopolysaccharide (LPS)-stimulated human intestinal cells. Methods: Intercellular adhesion molecule-1 (ICAM-1) expression was determined by Western blot analysis; interleukin-8 (IL-8) and tumor necrosis factor-¦Á (TNF-¦Á) concentrations were measured by enzyme-linked immunosorbent assays in Caco-2 cells 24 hours after treatment with LPS (1 ¦Ìg/mL) in presence of different concentrations of gelatin tannate. Results: ICAM-1 is induced on a wide variety of cells by inflammatory stimuli such as LPS. Our results have shown gelatin tannate as a potent inhibitor of ICAM-1 expression in LPS-stimulated Caco-2 cells. IL-8 and TNF-¦Á are important inflammatory mediators, recruiting neutrophils and T-lymphocytes. Together with LPS, adding gelatin tannate at different concentrations induced a dose-dependent inhibition of IL-8 and TNF-¦Á released by Caco-2 cells. Conclusion: These results suggest that gelatin tannate exerts anti-inflammatory effects by inhibiting the specific cytokines and adhesion molecules involved in several inflammatory disorders.
%K Caco-2
%K ICAM-1
%K IL-8
%K TNF-¦Á
%U https://www.dovepress.com/gelatin-tannate-reduces-the-proinflammatory-effects-of-lipopolysacchar-peer-reviewed-article-CEG