%0 Journal Article %T Bioremediation of Fluorophenols by Glycosylation with Immobilized Marine Microalga Amphidinium Crassum %A Kei Shimoda and Hiroki Hamada %J Environmental Health Insights %D 2012 %I %R 10.4137/EHI.S5392 %X Fluorophenols are used as agrochemicals and released into environment as pollutants. Cultured marine microalga Amphidinium crassum (Gymnodinium) glucosylated 2-fluorophenol (1), 3-fluorophenol (2), and 4-fluorophenol (3) to the corresponding ¦Â-D-glucosides, ie, 2-fluorophenyl ¦Â-D-glucoside (4, 60 ¦Ìg/g cells), 3-fluorophenyl ¦Â-D-glucoside (5, 20 ¦Ìg/g cells), and 4-fluorophenyl ¦Â-D-glucoside (6, 40 ¦Ìg/g cells). On the other hand, 2-, 3-, and 4-fluorophenols were efficiently converted by immobilized A. crassum in sodium alginate gel to give their ¦Â-D-glucosides in higher yields (4: 140 ¦Ìg/g cells; 5: 60 ¦Ìg/g cells; 6: 100 ¦Ìg/g cells). In repetitive batch use, the immobilized cells of A. crassum maintained the potential for the glucosylation of the substrate fluorophenol after 5 times of usage. %U http://www.la-press.com/bioremediation-of-fluorophenols-by-glycosylation-with-immobilized-mari-article-a2345