%0 Journal Article
%T Local Cell Proliferation Upon Enucleation in Direct Retinal Brain Targets in the Visual System of the Adult Mouse
%A Annemie Cuyvers
%A Melissa Paulussen
%A Katrien Smolders
%A Tjing-Tjing Hu and Lutgarde Arckens
%J Journal of Experimental Neuroscience
%D 2012
%I 
%R 10.4137/JEN.S4104
%X In this study we used incorporation of the DNA synthesis marker 5-bromo-2¡¯-deoxyuridine or BrdU to visualize cell proliferation in the visual system of the adult mouse as a response to monocular enucleation. We detected new BrdU-labeled cells in different subcortical retinal target regions and we established a specific time frame in which this cell proliferation occurred. By performing immunofluorescent double stainings for BrdU and different vascular (glucose transporter type 1, collagen type IV), glial (thymosin ¦Â4, glial fibrillary acidic protein) and neuronal (Neuronal Nuclei, doublecortin) markers, we identified these proliferating cells as activated microglia. Additional immunohistochemical stainings for thymosin ¦Â4 and glial fibrillary acidic protein also revealed reactive astrocytes in the different retinorecipient nuclei and allowed us to delineate a time frame for microglial and astroglial activation. A PCR array experiment further showed increased levels of cytokines, chemokines, growth factors and enzymes that play an important role in microglial-astroglial communication during the glial activation process in response to the deafferentation.
%U http://www.la-press.com/local-cell-proliferation-upon-enucleation-in-direct-retinal-brain-targ-article-a1825