%0 Journal Article
%T Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
%A Hongjun Jin
%A Yuehua Yu
%A William B. Chrisler
%A Yijia Xiong
%A Dehong Hu and Chenghong Lei
%J Breast Cancer: Basic and Clinical Research
%D 2012
%I 
%R 10.4137/BCBCR.S8513
%X Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness.
%U http://www.la-press.com/delivery-of-microrna-10b-with-polylysine-nanoparticles-for-inhibition--article-a2952