%0 Journal Article
%T Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR
%A Abhishek Chaturbedi
%A Liping Yu
%A Mark E Linskey and Yi-hong Zhou
%J Biomarker Insights
%D 2012
%I 
%R 10.4137/BMI.S9003
%X 1p/19q (1p and/or 19q) deletions are prognostic factors in oligodendroglial tumors (OT) and predict better survival after both chemotherapy and radiotherapy. While studying 1p/19q status as a potential variable within multivariate prognosis models for OT, we have frequently encountered unknown 1p/19q status within our glioma sample database due to lack of paired blood samples for loss of heterozygosity (LOH) assay and/or failure to perform fluorescence in situ hybridization (FISH). We realized that a 1p and 19q deletion assay that could be reliably performed solely on tumor DNA samples would allow us to fill in these molecular biology data ˇ°holesˇ±. We built recombinant DNA with fragments of the selected ˇ°markerˇ± genes in 1p (E2F2, NOTCH2), and 19q (PLAUR) and ˇ°referenceˇ± genes (ERC2, SPOCK1, and SPAG16 ) and used it as quantification standard in real-time PCR to gain absolute ratios of marker/reference gene copy numbers in tumor DNA samples, thus called comparative quantitative PCR (CQ-PCR). Using CQ-PCR, we identified 1p and/or 19q deletions in majority of pure low-grade oligodenroglioma (OG) tumors (17/21, 81%), a large portion of anaplastic oligodendroglioma (AO) tumors (6/15, 47%), but rarely found in mixed oligoastrcytomas (OA) tumors (1/8, 13%). These data are consistent with results of LOH and FISH assays generally reported for these tumor types. In addition, 15 out 18 samples showed concordant results between FISH and CQ-PCR. We conclude that CQ-PCR is a potential means to gain 1p/19q deletion information, which prognostic and predictive values of CQ-PCR-derived 1p/19q status will be determined in a future study.
%U http://www.la-press.com/detection-of-1p19q-deletion-by-real-time-comparative-quantitative-pcr-article-a3033