%0 Journal Article
%T The effect of oestradiol-17¦Â on the motility, viability and the acrosomal status of bull sperm
%A HB  iftci
%A U Z¨¹lkadir
%J South African Journal of Animal Science
%D 2010
%I South African Society for Animal Science
%X The aim of this study was to test the effect of oestradiol-17¦Â (E2) on the motility, viability and the acrosomal status of bull sperm in vitro. Pooled semen from Holstein bulls were incubated in the presence of 2, 4, and 8 ¦Ìg E2/mL for 24 h. Semen was also incubated in media without E2. During the incubation, the number of motile, viable sperm and the number of sperm possessing lost/damaged acrosomes, cytoplasmic droplets and coiled tails were counted at 0, 4, 18 and 24 h of incubation. Addition of 2 ¦Ìg E2/mL at 18 h of incubation increased the total motility over the control. The number of forward progressing sperm was increased by the supplementation of 2 and 8 ¦Ìg E2/mL over the control group at the 4 h incubation. Lower doses of E2 (2 and 4 ¦Ìg/mL) did not affect viability of sperm, but a high dose of E2 (8 ¦Ìg/mL) caused reductions in viability at 4 and 24 h of incubation. The number of sperm cells with lost acrosomes was significantly high in control group at 24 h of incubation. The number of sperm cells possessing proximal and distal cytoplasmic droplets and the number of sperm cells bearing coiled tails were not altered by any of the treatments. A small dose of E2 (2 ¦Ìg/mL) had a beneficial effect on the motility and acrosome integrity of bull sperm in vitro. Higher dose of E2 (8 ¦Ìg/mL) had a detrimental effect on viability.
%U http://www.ajol.info/index.php/sajas/article/view/54124