%0 Journal Article
%T In vitro Regeneration of Taverniera Abyssinica A. Rich: A Threatened Medicinal Plant
%A B Abera
%A L Negash
%A J Kumlehn
%A T Feyissa
%J Ethiopian Journal of Education and Sciences
%D 2011
%I Jimma University
%X In vitro germination, callus induction and plant regeneration has been established for dingetegna, Taverniera abyssinica. The best in vitro germination of seeds and vigorous seedlings growth as a prerequisite for the development of tissue culture methods was obtained on Murashige and Skoog medium supplemented with 12 g 1-1 phytoagar without sucrose. Light green compact calli from node, petiole and shoot meristem explants were efficiently induced on Gamborg medium containing 0.90 or 1.80 ¨¬M dichlorophenoxyacetic acid (2,4-D) combined with 2.22 ¨¬M6-benzylaminopurine (BAP), and supplemented with 30 g 1-1 sucrose and 5 g 1-1 phytagel. Callus initiation from shoot meristems and nodes was faster and occurred with a higher frequency than callus initiation from petiole and leaf segments (P<0.05). A high frequency of shoot regeneration (100%) was obtained upon transfer of calli onto regeneration medium containing 8.88 ¨¬M BAP combined with 1.14 ¨¬M indoleacetic acid (IAA). Regenerated shoots were transferred to rooting medium, which turned out to be optimal when half strength B5 medium was supplemented with 9.84 ¨¬M indolebutyric acid (IBA). Upon transfer to glasshouse, 86% survived and grew vigorously. The development of in vitro regeneration protocol for T. abyssinica provides the possibility to preserve endangered germplasm from the increasingly devastating man-made environmental conditions. Moreover, the method established can be used for micropropagation and  genetic improvement of this medicinally important species.Abbreviations: BAP ¨C -benzaylaminopurine; 2,4-D ¨C dichlorophenoxyacetic acid; IAA ¨C indole-3-acetic acid; IBA ¨C indole-3-butyric acid; NAA - a-naphthalenacetic acid; TDZ ¨C 1-phenyl-3-(1,2,3-thiadiazol-5-yl) ure.
%U http://www.ajol.info/index.php/ejesc/article/view/65381