%0 Journal Article %T Isolation of chlorophyll a from spinach leaves %A E.D. Dikio %A D.A. Isabirye %J Bulletin of the Chemical Society of Ethiopia %D 2008 %I Chemical Society of Ethiopia %X An efficient method for separating chlorophyll a from spinach leaves by column chromatography and solvent extraction techniques has been developed. The purity and identity of the chlorophyll a have been confirmed by UV-Vis, IR and mass spectrometry. Yields from 100 g of freeze-dried spinach were 23 ¨C 24 mg of chlorophyll a. KEY WORDS: Chlorophyll a, Spinach leaves, Separation, Column chromatography, Solvent extraction, Pheophytin a Bull. Chem. Soc. Ethiop. 2008, 22(2), 301-304. %U http://www.ajol.info/index.php/bcse/article/view/61302