%0 Journal Article
%T RNAi: An emerging field of molecular research
%A MH Kabir
%A SA Qadir
%A SHA Hassan
%A J Ahn
%A M Wang
%J African Journal of Biotechnology
%D 2008
%I Academic Journals
%X RNA silencing, named as co-suppression or post transcriptional gene silencing (PTGS) was found in transgenic plants which was the result of cellular mRNA degradation and silencing of gene expression. RNA interference (RNAi) is a specific technique using only a few double stranded RNA (dsRNA) molecules to stop the expression which has made it one of the important areas in molecular biology. By introducing a gene into the host genome which is highly homologous to an endogenous gene, the RNA silencing is initiated. Double-stranded RNA (dsRNA) is cut by the enzyme ¡°Dicer¡± producing small interfering RNAs (siRNAs) which combine with RNA-induced silencing complex (RISC). RISC, a protein complex, binds one strand of siRNA with mRNA of native target gene for destruction, resulting in gene silencing. The mechanism of RNAi offers a quick and easy way to determine the function of a gene. In this review, we discuss the history, components, mechanism and the application of RNA interference.
%U http://www.ajol.info/index.php/ajb/article/view/59671