%0 Journal Article
%T Two mini-preparation protocols to DNA extraction from plants with high polysaccharide and secondary metabolites
%A C S¨¢nchez-Hern¨¢ndez
%A JC Gayt¨¢n-Oyarz¨²n
%J African Journal of Biotechnology
%D 2006
%I Academic Journals
%X Were standardized two previously reported standard plant DNA extraction methods, but improved them on mini preparations to use the samples for population genetic analysis. The combination of CTAB lysis procedure-solvent extraction and DNA column purification (DNeasy plant mini kit modification) enables a faster and reliable DNA extraction from all samples tested:   Piper, Quercus, Zea and cacti species (considered ¡°hard¡± extraction species), and this protocol uses smaller tissue samples than other mini or midi-prep protocols. We obtained high quality and DNA yields in all samples tested. This alternative protocol (CTAB lysis-solvent extraction based) is an excellent option if there are many samples to process and it is also a non expensive protocol. This method also produces good DNA quality but fewer yields. Both two protocols produce reproducible PCR pattern-bands amplification with all the genetic markers tested (RAPD¡¯s and microsatellites). The DNA obtained was used in other molecular biology standard analysis methods, like enzymptic restriction patterns, ligation, sequencing and cloning with good results too.
%U http://www.ajol.info/index.php/ajb/article/view/55890