%0 Journal Article
%T Molecular cloning and characterization of a new peroxidase gene (OvRCI) from Orychophragmus violaceus
%A D Qilin
%A W Jin
%A F Bin
%A L Tingting
%A C Chen
%A L Honghui
%A D Shizhang
%J African Journal of Biotechnology
%D 2009
%I Academic Journals
%X A new peroxidase gene from Orychophragmus violaceus was cloned. The full-length cDNA of O.violaceus peroxidase gene (OvRCI, GenBank. Acc. No. AY428037) was 1220 bp and contained an 1128 bp open reading frame encoding a protein of 375 amino acids. Homology analysis and molecular modeling revealed that OvRCI strongly resembled other peroxidase genes. Quantitative real-time PCR analysis revealed that it was a constitutively salt-inducible gene and its transcript level was most abundant after 24 h treatment with 200 mmol.L-1 sodium chloride. Our studies suggested that OvRCI was a new member of the family of recently cloned peroxidase genes.
%U http://www.ajol.info/index.php/ajb/article/view/66176