%0 Journal Article
%T Molecular Reassessment Of Relationships Within Vicieae Using Electrophoretic And Immunochemical Techniques
%A R H Sammour
%J African Crop Science Journal
%D 2005
%I 
%X An array of electrophoretic and immunochemical techniques was used to investigate legumins, vicilins, and albumins of seed storage proteins of pea (Pisum sativum), bean (Vicia faba), lentil (Lens esculentum) and chick pea (Cicer arientinum) to delimit the boundary of the tribe Vicieae and to clarify the systematic position of the genus Cicer. The band patterns of the legumins of these species were broadly similar in having bands at Mr 60,000 under non-reducing conditions and two sets of bands, at Mr approximately 40,000 and 20,000 under reducing conditions. And the band patterns of the vicilins were quit similar showing bands at Mr approximately 71,000 (convicilin) and 50,000 (vicilin). Serologically, the legumins of V. faba and L. esculentum gave a total identity with P. sativum legumin antiserum under non-reducing conditions, whereas the legumin of C. arientinum exhibited a partial identity, which was attributed to the failure of the low molecular sub-unit pair (Mr 33,000) to react with Pisum legumin antiserum. The vicilins of V. faba, L. esculentum and C. arientinum had a partial identity with vicilin of Pisum sativum. The electrophoretic patterns of V. faba, L. esculentum and C. arientinum albumins were markedly different. However, they gave a positive reaction with Pisum major albumin antiserum (Mr 25000). Immunochemically, the vicilin and legumin of Cicer were more related to those of Vicieae than to those of Phaseoleae, Glycineae, Cajaneae, Diocleae. Thus, the data presented in this work suggested the classification of Cicer under Vicieae rather than a separate tribe Cicerideae. Key Words: Immunodiffusion, legumins, tribe Vicieae, vicilins, Western blotting RESUME Une s¨¦lection des techniques ¨¦lectrophor¨¦tique et immunochimiques ¨¦tait utilis¨¦e pour examiner les l¨¦gumines, vicilines, et albumines des prot¨¦ines des graines de petit pois (Pisum sativum), haricot (Vicia faba), lentille (Lens esculentum) et culin (Cicer arientinum) pour limiter les fronti¨¨res de la tribu Vicieae et pour clarifier la position syst¨¦matique de la vari¨¦t¨¦ Cicer. Les bandes prototypes des l¨¦gumines de ces esp¨¨ces ¨¦taient g¨¦n¨¦ralement similaires en ayant des bandes ¨¤ Mr 60.000 sous les conditions de non att¨¦nuation et deux groupes des bandes, ¨¤ Mr 40.000 et 20.000 approximativement sous les conditions d\'att¨¦nuation. Et les bandes prototypes des vicilines ¨¦taient un peu similaires montrant des bandes ¨¤ Mr 71.000 (conviciline) et 50.000 (viciline) approximativement. S¨¦rologiquement, les l¨¦gumines de V. faba et L. esculentum ont donn¨¦ une identit¨¦ totale avec la l¨¦gumine antis¨¦rum de P. sativum sous les conditions de non att¨¦nuation, tandis que les l¨¦gumines de C. arientinum ont exhib¨¦ une identit¨¦ partielle, laquelle, ¨¦tait attribu¨¦e ¨¤ l\'¨¦chec de la sous unit¨¦ paire mol¨¦culaire basse (Mr 33.000) de r¨¦agir avec le Pisum sativum antis¨¦rum. Les vicilines de V. faba, L. esculentum et C. arientinum avaient une identit¨¦ partielle avec la viciline de Pisum sativum. Les prot
%U http://www.ajol.info/index.php/acsj/article/view/27636