%0 Journal Article
%T Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris
%A HA C¨®rdoba-Ruiz
%A RA Poutou-Pi ales
%A OY Echeverri-Pe a
%A NA Algecira-Enciso
%A P Land¨˘zuri
%A H S¨˘enz
%A LA Barrera-Avellaneda
%J African Journal of Biotechnology
%D 2009
%I Academic Journals
%X Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDSLike) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. The biological activity found was between 7.3 and 29.5 nmol h-1 mg-1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same clone in shake flask culture.
%U http://www.ajol.info/index.php/ajb/article/view/60382