%0 Journal Article
%T Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas
%A J. Soko owska
%A J. Micu¨½
%A K. Zabielska
%A E. Malicka
%A R. Lechowski
%J Polish Journal of Veterinary Sciences
%D 2010
%I 
%R 10.2478/v10181-010-0004-5
%X Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (¦Ê and ¦Ë) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Expression of ¦Ë light chains and ¦Ê light chains was observed in 18/19 and 1/19 tumors, respectively. In the majority of neoplastic cells in each examined specimen this reaction had a membranous pattern (s¦Ê/s¦Ë). In all examined cases the presence of immunoglobulin light chains was also observed in the cytoplasm of some neoplastic cells (c¦Ê/c¦Ë). These cells were usally rare and never constituted a dominant population. The expression of immunoglobulin was found in 13/19 cases. Most lymphomas were sIgM positive (11/13 cases). In one case expression of IgG was found, and in another lymphoma two populations of neoplastic cells with different expression of examined immunoglobulins (cells with IgM+ and IgG+ phenotypes) were observed. The reaction also had a membranous pattern. The cells containing cytoplasmic immunoglobulins were rare, and in most cases were of the same type as the surface immunoglobulins. Our study has confirmed that canine lymphomas are a monoclonal proliferation of B-cells usually expressing immunoglobulin ¦Ë light chains and that the vast majority of tumors deriving from B-cells express IgM. Our study also indicates a possibility of occurence of biclonal lymphomas in canine species.
%K dog
%K lymphoma
%K immunoglobulin light chains
%K immunoglobulin
%U http://versita.metapress.com/content/83p354v181h31n05/?p=88b9e8e23eef43c8a41d44799928e68e&pi=6