%0 Journal Article
%T Tumor Necrosis Factor-alpha as a possible auto-/paracrine factor affecting estrous cycle in the cat uterus
%A M. J. Siemieniuch
%A K. Ogrodowska
%A H. Ohgawara
%A D. J. Skarzynski
%A K. Okuda
%J Polish Journal of Veterinary Sciences
%D 2010
%I 
%R 10.2478/v10181-010-0017-0
%X Tumor Necrosis Factor-alpha (TNF¦Á) is a pleiotrophic cytokine, affects either normal or tumor cells, and influences cellular differentiation. TNF¦Á role in female reproduction has been proven to be mediated through an influence on prostaglandin (PGs) synthesis and output. To evaluate the possible role of TNF¦Á in an auto-/paracrine regulation in the cat uterus, mRNA expression coding for TNF¦Á and its receptors (TNFR1 and TNFR2), and TNF¦Á protein content at different stages of the estrous cycle were investigated. Additionally, TNF¦Á involvement in PG secretion at different stages of the estrous cycle was investigated by in vitro tissue culture. Gene expressions coding for TNF¦Á and TNFR1 were the highest at diestrus (P <0.05). TNF¦Á protein expression was the lowest at interestrus (P <0.05). Nevertheless, TNFR2 was not affected by the estrous stage. TNF¦Á at a dose of 1 ng/ml significantly increased PGF2¦Á secretion at estrus (P <0.01) and PGE2 secretion at diestrus (P <0.001) after 12h incubation. Overall findings indicate that TNF¦Á locally produced in the cat's uterus, stimulates PG secretion in an estrous cycle-related manner.
%K Tumor Necrosis Factor-alpha
%K prostaglandin
%K uterus
%K cat
%U http://versita.metapress.com/content/72239506421760q2/?p=88b9e8e23eef43c8a41d44799928e68e&pi=4