%0 Journal Article
%T The effect of progesterone on oxytocin-stimulated intracellular Ca2+ mobilisation and prostaglandin secretion in porcine endometrium
%A G. Kotwica
%A A. Oponowicz
%A B. Kurowicka
%A A. Franczak
%A I. Bogacka
%J Polish Journal of Veterinary Sciences
%D 2010
%I 
%R 10.2478/v10181-010-0012-5
%X We have studied in the porcine endometrium the expression of oxytocin receptor (OTR) mRNA and the effect of progesterone (P4) on oxytocin/oxytocin receptor (OT/OTR) function concerning intracellular Ca2+mobilisation ([Ca2+]i), prostaglandin F2¦Á (PGF2¦Á) and E2 (PGE2; PG) secretion. Tissue was taken from cyclic and early pregnant pigs (days 14-16). A higher expression of OTR mRNA (P<0.05) was observed in the endometrium of cyclic than pregnant pigs. The stimulatory (P<0.05) effect of OT (10-7 M) on [Ca2+]i mobilisation was noticed within 15-60 s and 30-60 s in endometrial stromal cells of cyclic and pregnant pigs, respectively. In the presence of P4 (10-5 M) basal and OT-stimulated [Ca2+]i concentrations decreased in stromal cells during luteolysis and pregnancy. In stromal cells P4 delayed mobilisation of [Ca2+]i in response to OT by 15 s during luteolysis and had no effect during pregnancy. In cyclic and pregnant epithelial cells OT stimulated mobilisation of [Ca2+]i in 45 s and 60 s, respectively. Oxytocin increased (P<0.05) PGF2¦Á secretion during luteolysis and pregnancy and PGE2 during luteolysis from endometrial slices. Progesterone did not inhibit this stimulatory effect. During luteolysis OT increased (P<0.05) PGF2¦Á in epithelial and stromal cells and PGE2 secretion in epithelial cells. In the presence of P4 this effect of OT was reduced only in stromal cyclic cells (6 h culture). The presence of P4 decreased the effect of OT on [Ca2+]i mobilisation only in stromal cells. We found that, in most conditions, P4 did not inhibit the OT-stimulated secretion of PG in the porcine endometrium.
%K pigs
%K oxytocin
%K prostaglandins
%K luteolysis
%K pregnancy
%U http://versita.metapress.com/content/yw61r3028615308n/?p=88b9e8e23eef43c8a41d44799928e68e&pi=5