%0 Journal Article
%T Setting up a gene expression study for tissue cells by method of quantitative real-time PCR
%A Daliborka Du ani
%A Luka Bolha
%A Mojca Narat
%A Irena Oven
%J Acta agriculturae Slovenica
%@ 1854-1941
%D 2012
%I University of Ljubljana
%R 10.2478/v10014-012-0009-0
%X Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings.
%K molecular genetics
%K RT-qPCR
%K gene expression
%K tissue cells
%U http://versita.metapress.com/content/t43172368w696300/?p=9e7075bd05cc4181af1f7e7ae0cbe7cf&pi=1