%0 Journal Article %T Growth Regulators Effects on In Vitro Shoot Regeneration of Sainfoin (Onobrychis sativa Lam.) %A S. Saglam %J Biotechnology & Biotechnological Equipment %D 2010 %I %R 10.2478/V10133-010-0089-0 %X The effect of different concentrations of BAP (6-benzylaminopurine)-NAA (a-naphthaleneacetic acid) on in vitro propagation from cotyledon node explant of sainfoin (Onobrychis sativa Lam.) was studied. The developed large numbers of shoots were propagated from explants within 6 weeks on Murashige and Skoog (MS) media. The longest shoot length (6.79 cm) was recorded on MS medium containing 1 mg/l BAP, 0.02 mg/l NAA. The observed difficulties were overcome after pulse treatment with 50 mg/l IBA for 10 min on MS medium for rooting. %K Onobrychis sativa %K Sainfoin %K shoot regeneration %K cotyledon node %K BAP %K NAA %U http://versita.metapress.com/content/0k51m21uj80u7505/?p=e877066f55704595b4a4c5ea69fc4b70&pi=5