%0 Journal Article
%T Biotinylated Microbubbles Targeted to Amyloid
%A Arlymae Rand
%A Gregory Gilman
%A Dennis J. O¡¯Kane and Marek BelohlavekBackground:Methods:Results:Conclusion:
%J The Open Clinical Chemistry Journal
%D 2008
%I Bentham Open
%R 10.2174/1874241600801010075]
%X Background: Ultrasonography uses microbubbles for enhanced imaging. We created microbubbles that have preferential adherence to amyloid protein by utilizing the affinity of serum amyloid component P (SAP) to amyloid along with avidin-biotin interactions. Methods: Biotin-labeled albumin was incorporated into the albumin shell of fluorocarbon gas-filled bubbles. The bubble was attached through a bridge with biotin incorporated into the shell of the bubble and incubated with avidin-labeled SAP which was pre-bound to SA (¡°synthetic amyloid¡±). This resulted in a bubble targeted to amyloid. The bubbles were evaluated using fluorescent microscopy and fluorescence-activated cell sorting (FACS). Results: Microbubbles with a protein shell were bound to amyloid by utilizing the affinity of SAP for amyloid and biotinavidin interactions. Conclusion: We introduce microbubbles specifically targeted to amyloid deposits and intended as future mediators of ultrasound detection of amyloid deposits and amyloid-selective drug delivery.
%U http://www.benthamscience.com/open/tocchemj/articles/V001/75TOCCHEMJ.htm