%0 Journal Article
%T Research at channel level on the effect of LaCl3 on Radish (Raphanus satirus L.) vacuolar membrane
%A Yanfang Shang
%A Pin Yang
%A Zhenming Pei
%J Chinese Science Bulletin
%@ 1861-9541
%D 2002
%I 
%R 10.1360/02tb9025
%X We recorded slow vacuolar (SV-type) channel currents of Radish vacuoles successfully for the first time by using the whole-vacuolar patch-clamp recording mode. SV-type currents would increase and threshold potentials of activation would shift towards more negative values with the increase of concentrations of cytosolic Ca2+. When 2.5 mmol/L LaCl3 and 4 mmol/L EGTA were added to bath solutions, SV-type currents were suppressed remarkably. Then adding LaCl3 with different concentrations to pipette solutions, we found that LaCl3 with higher concentrations (>4 กม 10 7 mol/L) had a strong inhibitory effect on SV-type currents, while LaCl3 with lower concentrations ( 4 กม 10 7 mol/L) promoted channel currents. This promoting effect provides an important basis at channel level for researching further the effects of rare earth on physiological activities of plants and the production-increase effects of rare earth fertilizers on crops.
%K whole-vacuolar patch-clamp recording
%K SV-type channel current
%K cytosolic Ca2+
%K La3+
%U http://link.springer.com/article/10.1360/02tb9025