%0 Journal Article %T Two-dimensional crystallization and preliminary electron crystallographic result of partially purified F0 from porcine mitochondria %A Xujia Zhang %A Jingchuan Sun %A Hui Yang %A Huilin Li %A Youguo Huang %A Fuyu Yang %J Chinese Science Bulletin %@ 1861-9541 %D 2002 %I %R 10.1360/02tb9077 %X After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembrane sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitution of the partially purified F0 into asolectin and microdialysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a=b=14.4 nm. A unit cell contains four F0 molecules. %K membrane protein %K proton channel F0 %K two-dimensional crystal %K electron microscopy %U http://link.springer.com/article/10.1360/02tb9077