%0 Journal Article
%T Crystal structure of thermostable catechol 2,3-dioxygenase determined by multiwavelength anomalous dispersion method
%A Tao Jiang
%A Chaoneng Ji
%A Xiaoyu Sheng
%A Mingqin Chen
%A Yi Xie
%A Weimin Gong
%A Yumin Mao
%J Chinese Science Bulletin
%@ 1861-9541
%D 2002
%I 
%R 10.1360/02tb9073
%X The selenomethionyl derivative of the thermostable catechol 2,3-dioxygenase (SeMet-TC23O) is expressed, purified and crystallized. By using multiwave length anomalous dispersion (MAD) phasing techniques, the crystal structure of TC23O at 0.3 nm resolutions is determined. TC23O is a homotetramer. Each monomer is composed of N-terminal and C-terminal domains (residues 1กซ153 and 153กซ319, respectively). The two domains are proximately symmetric by a non-crystallographic axis. Each domain contains two characteristic motifs which are found in almost all of extradial dioxygenases.
%K multiwavelength anomalous dispersion (MAD)
%K X-ray diffraction
%K thermostable catechol 2
%K 3-dioxygenase
%K crystal structure
%K synchrotron light source
%U http://link.springer.com/article/10.1360/02tb9073