%0 Journal Article
%T Identification of a novel DNA molecule associated with Tobacco leaf curl virus
%A Yan Xie
%A Xueping Zhou
%A Zhenghe Li
%A Zhongkai Zhang
%A Guixin Li
%J Chinese Science Bulletin
%@ 1861-9541
%D 2002
%I 
%R 10.1360/02tb9282
%X A circular DNA molecule, designated as DNA¦Â, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNA¦Â sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNA¦Â of Y5 and Y8 (TLCV DNA¦Â) were determined. Y5 DNA¦Â comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNA¦Â consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNA¦Â has little sequence homology to DNA-A of TLCV., except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNA¦Â shared 85% sequence homology with Y8 DNA¦Â, and both Y5 DNA¦Â and Y8 DNA¦Â had relatively low sequence identity (51%¨C65%) with the reported DNA¦Â molecules associated with Ageratum yellow vein virus and Cotton leaf curl virus. The immunotrapping PCR and whitefly transmission tests showed that DNA¦Â molecule could be encapsidated in virus particle and transmitted by Bemisia tabaci. This is the first report of DNA¦Â associated with WTGs in China.
%K DNA¦Â
%K Tobacco leaf curl virus
%K sequence
%K Begomovirus
%K Bemisa tabaci
%U http://link.springer.com/article/10.1360/02tb9282