%0 Journal Article
%T Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta)
%A Peng Jiang
%A Song Qin
%A Chengkui Tseng
%J Chinese Science Bulletin
%@ 1861-9541
%D 2002
%I 
%R 10.1360/02tb9317
%X A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the microparticle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529 ¦Ìg/mg soluble proteins on average and the highest value was 2.497 ¦Ìg/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR-Southern and total DNA hybridization. Prospect of kelp bioreactor producing high value materials such as edible HBV vaccine was discussed as well.
%K Laminaria japonica
%K genetic engineering
%K hepatitis B surface antigen (HBsAg)
%K expression system
%K vaccine
%U http://link.springer.com/article/10.1360/02tb9317