%0 Journal Article
%T Inhibitory effect of a modified adenovirus type 5E1A gene on the NF-百B activity in porcine aortic endothelial cells induced by TNF-汐
%A Yewei Ma
%A Xiaoshan Zhou
%A Qingzheng Zhao
%A Jun Yang
%A Xin Gao
%A Yanchun Li
%A Yuying Liu
%A Zheng Wang
%J Chinese Science Bulletin
%@ 1861-9541
%D 2003
%I 
%R 10.1007/BF03183340
%X The transcription factor nuclear factor 百B (NF-百B) plays a key role in the delayed xenograft rejection (DXR). One of the important objects in the field is how to inhibit the NF-百B activity at optimal level. Thus, a modifiedE1A gene (E1A忖) containing function domain (1每80 aa) and nuclear localization domain (139每243 aa) was used and cloned into an eucaryotic expression vector pcDNA3 to transfect the porcine aortic endothelial cells (PAEC). The stable transfectants were screened with G418.E1A忖 gene was able to be stably expressed in the PAEC and could not affect the growth of PAEC as analyzed by RT-PCR and cell growth rate. Reporter gene assay demonstrated thatE1A忖 was capable of inhibiting NF-百B activity in the PAEC induced by TNF-汐 without sensitizing to apoptosis, and the rate of inhibition was 53%. Furthermore,E1A忖 inhibited the expression of a NF-百B-dependent inflammatory geneE-selectin in the cells, and the rate of inhibition was 63%. In summary, the usage ofE1A忖 gene may be a new strategy to overcome DXR in the xenotransplantation.
%K delayed xenograft rejection
%K E1A gene
%K porcine aortic endothelial cells
%K NF-B
%K E-selectin
%U http://link.springer.com/article/10.1007/BF03183340