%0 Journal Article
%T Preparation of the selenomethionine derivative of tabtoxin resistance protein
%A Hongzhen He
%A Yu Shao
%A Chen Yuhang
%A Liu Bingbing
%A Cao Zhenbo
%A Jiang Fan
%A Liu Yiwei
%A Tang Hong
%A Liu Jinyuan
%A Zhao Nanming
%A Rao Zihe
%J Chinese Science Bulletin
%@ 1861-9541
%D 2001
%I 
%R 10.1007/BF03184331
%X In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed inE. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.
%K Se-Met TTR derivative
%K expression
%K purification
%K mass spectrometry
%U http://link.springer.com/article/10.1007/BF03184331