%0 Journal Article
%T Analysis of nucleosome positioning in promoters of miRNA genes and protein-coding genes
%A HongDe Liu
%A DeJin Zhang
%A JianMing Xie
%A ZhiDong Yuan
%A Xin Ma
%A ZhiYuan Lu
%A LeJun Gong
%A Xiaoauthor Sun
%J Chinese Science Bulletin
%@ 1861-9541
%D 2010
%I 
%R 10.1007/s11434-009-3730-2
%X Nucleosome positioning in promoters is important for gene transcription regulation. In this paper, with a nucleosome prediction model, curvature profile, the characteristics of nucleosome positioning in promoters are analyzed for miRNA genes and protein-coding genes. In the vicinity of transcription start site (TSS), there is a nucleosome-free region (NFR) followed by a positioned nucleosome at ¡«200 bp downstream of TSS. A similar characteristic is observed in independent intronic promoters and intergenic promoters, namely, both types of promoters have a longer NFR in 0¨C 400 bp upstream of TSS. Moreover, transcription factor binding sites (TFBSs) locate in the NFR with a high concentration. However, nucleosome pattern in dependent intronic promoters are like that in protein-coding promoters, with two nucleosomes positioned at  200¨C 400 bp and  400¨C 600 bp upstream of TSS. The results indicate nucleosome positioning is probably different in independent miRNA promoters and protein-coding promoters; and positioning seems to be an important factor not only in regulation of protein-coding gene, but also in that of miRNA gene.
%K nucleosome positioning
%K promoter
%K miRNA gene
%U http://link.springer.com/article/10.1007/s11434-009-3730-2