%0 Journal Article
%T Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR
%A An Yan
%A GuoHui Ding
%A ZhenFeng Zhou
%A Hui Dong
%A YaKun Zhang
%A Lei Zhu
%A YunGang He
%A GuoQing Zhang
%A YiXue Li
%A Bing Sun
%A Zhong Huang
%A Ke Lan
%A Li Jin
%A HongYan Wang
%A XiaoNing Wang
%A Zhong Yang
%A Yang Zhong
%A JianXin Dai
%A YaJun Guo
%A Hao Wang
%A XiaoYan Che
%A Fan Wu
%A ZhenGan Yuan
%A Xi Zhang
%A ZhiWei Cao
%A XiaoNong Zhou
%A JiaHai Zhou
%A ZhiYong Ma
%A GuangZhi Tong
%A GuoPing Zhao
%A WeiRong Jin
%A Hui Xiong
%J Chinese Science Bulletin
%@ 1861-9541
%D 2009
%I 
%R 10.1007/s11434-009-0433-7
%X The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin (HA) of several subtypes (including H1, H2, H3, H5, H7 and H9) were designed. Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential.
%K influenza A virus
%K HA gene
%K H1 and H3 subtypes
%K specific amplification by RT-PCT
%K DNA sequencing
%U http://link.springer.com/article/10.1007/s11434-009-0433-7