%0 Journal Article
%T Nutrient improvement for simultaneous production of exopolysaccharide and mycelial biomass by submerged cultivation of Schizophyllum commune AGMJ-1 using statistical optimization
%A Mayur Joshi
%A Harshad Patel
%A Shilpa Gupte
%A Akshaya Gupte
%J 3 Biotech
%@ 2190-5738
%D 2013
%I Springer
%R 10.1007/s13205-012-0103-3
%X Exopolysaccharides (EPS) of fungal origin have attracted special attention from researchers due to their multifarious applications in the food and pharmaceutical industries. In the present study, optimization of the process parameters for the production of exopolysaccharide by Schizophyllum commune AGMJ-1 was studied using one factor at a time (OFAT) method, Plackett¨CBurman design (PBD) and response surface methodology (RSM). OFAT method revealed xylose and yeast extract to be the most effective carbon and nitrogen sources and pH 5.3 as an optimum for maximum EPS production. Xylose, yeast extract and KCl were screened as statistically significant variables for EPS production using PBD. RSM based on the central composite design estimated that maximum EPS (4.26 g L 1), mycelial biomass (14 g L 1) and specific yield (0.45 g g 1) were obtained when concentration of xylose, yeast extract and KCl were set at 2.5 g % (w/v), 0.83 g % (w/v) and 6.53 mg % (w/v), respectively, in the production medium.
%K Exopolysaccharide
%K Mycelial biomass
%K Schizophyllum commune
%K One factor at a time
%K Plackett¨CBurman design
%K Response surface methodology
%U http://link.springer.com/article/10.1007/s13205-012-0103-3