%0 Journal Article
%T Human RBCs blood group conversion from A to O using a novel ¦Á-N-acetylgalactosaminidase of high specific activity
%A ChengYu Yu
%A Hua Xu
%A LiSheng Wang
%A JianGeng Zhang
%A YangPei Zhang
%J Chinese Science Bulletin
%@ 1861-9541
%D 2008
%I 
%R 10.1007/s11434-008-0248-y
%X ¦Á-N-acetylgalactosaminidase (¦ÁNAGA) can convert group A human red blood cells (RBCs) to group O. One novel ¦ÁNAGA gene was cloned by PCR from Elizabethkingia meningosepticum isolated from a domestic clinical sample. Pure recombinant ¦ÁNAGA was obtained by genetic engineering and protein purification with a calculated molecule of 49.6 kD. ¦ÁNAGA was selective for terminal ¦Á-N-acetylgalactosamine residue with a high specific activity. ¦ÁNAGA could completely remove A antigens of 1 U (about 100 mL) group A1 or A2 RBCs in 1 h at pH 6.8 and 25ˇăC with a consumption of 1.5 or 0.4 mg recombinant enzyme. Enzyme-converted group A RBCs did not agglutinate after being mixed with monoclonal anti-A or sera of groups A, B, AB and O. Other blood group antigens except ABO had no change. FCM analysis showed that A antigens and A1 antigens disappeared while H antigens increased. It indicated that ¦ÁNAGA successfully converted human blood group A RBCs to universally transfusable group O RBCs without the risk of ABO-incompatible transfusion reactions. This ¦ÁNAGA was suitable for producing universal RBCs to increase clinical transfusion safety, improve the RBCs supply, and to decrease transfusion cost and support transfusion service in case of emergency.
%K universal RBCs
%K blood group conversion
%K ¦Á-N-acetylgalactosaminidase
%K transfusion
%K A antigen
%K A1 antigen
%U http://link.springer.com/article/10.1007/s11434-008-0248-y