%0 Journal Article
%T Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation
%A Ramona Lieder
%A Sigr赤dur Th車ra Reynisd車ttir
%A Finnbogi Thorm車dsson
%A Chuen-How Ng
%A Jon Magn迆s Einarsson
%A J車hannes G赤slason
%A J車hannes Bj rnsson
%A Sveinn Gudmundsson
%A P谷tur Henry Petersen
%A 車lafur Eysteinn Sigurj車nsson
%J Natural Products and Bioprospecting
%@ 2192-2209
%D 2012
%I Springer
%R 10.1007/s13659-012-0017-0
%X Human mesenchymal stem cells (hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins (CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes.
%K YKL-40
%K mesenchymal stem cells
%K osteogenic differentiation
%K chitinase-like-protein
%U http://link.springer.com/article/10.1007/s13659-012-0017-0