%0 Journal Article
%T Real time monitoring of nucleic acids ligation based on molecular beacon
%A Zhiwen Tang
%A Kemin Wang
%A Weihong Tan
%A Jun Li
%A Lingfeng Liu
%A Qiuping Guo
%A Xiangxian Meng
%A Shasheng Huang
%A Du Li
%A Weifang Luo
%J Chinese Science Bulletin
%@ 1861-9541
%D 2003
%I 
%R 10.1007/BF03183940
%X A novel method has been developed to monitor the nucleic acids ligation process. Molecular beacon was employed here to convert the ligation information into fluorescence signal quickly and quantitatively. This method provides effective and original approach to researching the dynamic ligation process and the interactions between nucleic acids and ligase. An analytical method for T4 DNA ligase based on this way has been built up with a linear detection range from 2.310 4 U/mL to 0.23 U/raL. It is rapid and sensitive to detect 2.8 กม10 5 U T4 DNA ligase in 10 min.
%K nucleic acids ligation
%K T4 DNA ligase
%K real time
%K assay
%K molecular beacon
%U http://link.springer.com/article/10.1007/BF03183940